| Local | Visita | Hora | |
|---|---|---|---|
| Viernes 09 de Abril | |||
| San Jose Earthquakes | 3 - 2 | Oakland Roots | 12:00 |
| Orlando City SC | 3 - 2 | Minnesota Stars FC | 13:00 |
| Independiente del Valle | 2 - 1 | Gremio | 17:15 |
| Birmingham Legion | CANC | Nashville SC | 19:00 |
| Manta FC | 0 - 2 | Tecnico Universitario | 17:00 |
| Platense | 2 - 4 | San Lorenzo | 19:00 |
| Huila | 4 - 1 | Real Cartagena | 15:30 |
| Valledupar | 1 - 2 | Union Magdalena | 15:30 |
| Durango | 4 - 0 | Mazorqueros | 20:00 |
| Portimonense | 3 - 0 | Guimaraes | 14:00 |
| Hamburger SV | 1 - 2 | SV Darmstadt 98 | 11:30 |
| Arminia Bielefeld | 1 - 0 | SC Freiburg | 13:30 |
| Vitesse | 0 - 0 | ADO Den Haag | 13:00 |
| Huesca | 3 - 1 | Elche | 14:00 |
| Fulham | 0 - 1 | Wolves | 14:00 |
| Roda | 0 - 0 | Almere City FC | 10:00 |
| Metz | 0 - 2 | Lille | 14:00 |
| NAC Breda | 3 - 1 | Jong Utrecht | 11:00 |
| Den Bosch | 3 - 5 | Cambuur | 13:00 |
| Excelsior | 2 - 1 | NEC Nijmegen | 13:00 |
| FC Eindhoven | 0 - 2 | Jong PSV | 13:00 |
| FC Volendam | 5 - 1 | Helmond Sport | 13:00 |
| Reggiana | 0 - 1 | Empoli | 14:00 |
| Necaxa | 0 - 1 | U.N.A.M. - Pumas | 19:30 |
| FC Juarez | 2 - 1 | Atletico San Luis | 21:30 |
| Watford | 2 - 0 | Reading | 13:45 |
